Master Degree

Laboratory of Time Resolved Spectroscopies

Year of erogation: 
Unit Coordinator: 
Disciplinary Sector: 
Applied Physics (Cultural Heritage, Environment, Biology and Medicine)
Second semester
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Learning outcomes of the course unit

1. Knowledge and understanding
Understand the fundamental properties of the electronic states of organic molecules and the transitions between them and the experimental techniques discussed within the course according to the program reported.
Remember and compare the time-resolution characteristics of the spectroscopic techniques described in the lessons and the processes that they allow to follow in real time.
Explain the meaning of the physical quantities and the general concepts related with the spectroscopic techniques
Examine physical systems to which the methodologies addressed can be applied
Understand context and basic concepts of recent literature that use time-resolution spectroscopy techniques

2. Applying knowledge and understanding
Apply the acquired knowledge to address the photophysical processes of a molecular system
Identify the appropriate techniques for the study of a particular photophysical process
Perform numerical analysis of experimental signals and derive important physical parameters for the characterization of the underlying phenomenon
Elaborate reaction patterns from experimental data
3. Communication skills
Know how to communicate ideas-problems-solutions about photophysics processes in a clear, synthetic and effective way
Know how to describe the phases of a measurement appropriately
Able to explain to the group mates and the teacher the various experimental issues faced during laboratory practice

4. making judgements
Able to analyze from a quantitative point of view relevant photophysical processes both at the theoretical and experimental level
Able to evaluate the limits and potentials of the spectroscopic techniques described during the course.
Know how to evaluate the essential elements to develop a mechanistic model that describes a molecular process
Able to evaluate critically the validity limits of the developed models, and the analogies and differences between the physical systems studied
5. Learning Skills
Link the different topics discussed in the course and those dealt with in other courses (eg Physics of Matter, Quantum Mechanics, Chemistry)
To explore the main optical spectroscopic methodologies endowed with time resolution covered in cited scientific publications
Know how to change your conceptual framework or the plan of an experimental measure in response to new inputs by developing alternative solutions and methodologies


Electromagnetism, classical geometrical and wave optics, quantum mechanics, condensed matter physics

Course contents summary

This course intends to explore the foundations of selected spectroscopic methods normally used to investigate molecular properties. Ground and excited state properties as well as their interactions with the environment will be dealt with, with steady state and time resolved methodologies. The spectroscopic techniques dealt with in this course are endowed with time resolution which is adequate for studying transient or unstable molecular states.

Radiation-Matter interactions
Time resolved optical methods
Excited state processes
Fluorescence spectroscopy methods
Mechanisms and dynamics of competitive de-excitation
Photothermal spectroscopies
Single molecule detection
Laboratory practice

Course contents

Radiation-Matter interactions
Steady state optical methods
Time resolved optical methods
Excited state processes
Time resolved absorption
Pump and probe methods
Laser flash photolysis
Fluorescence spectroscopy methods
Time-Correlated Single-Photon Counting
Frequency-Domain detection
Fluorescence Correlation Spectroscopy
Mechanisms and dynamics of competitive de-excitation
Photothermal spectroscopies
Single molecule detection
Laboratory practice

Recommended readings

Principles of Fluorescence Spectroscopy, Third Edition, Joseph R. Lakowicz, Springer
Original research papers and reviews published on scientific journals

Teaching methods

In the event that the health situation allows the lessons and the practice to be held in person, the following didactic methodology will be followed.
The course consists in a series of lectures covering the fundamentals of the spectroscopic methodologies. For those methods available at this Department, laboratory practice will be taken.
Students will have to perform three experiments on molecular systems, that enable to follow relevant photophysical processes.
In the event that the health situation prevents the lessons or the labs from taking place in person, the lessons will be delivered in synchronous online mode, and the labs will be replaced by data analysis sessions on real experimental data previously collected.

Assessment methods and criteria

Assessment of the level of learning will be done on the basis of the relationships that the students, divided into groups, will disseminate on the exercises carried out.
Each report will be discussed with the workgroup components.
In the event that the health situation prevents the carrying out of the examination tests in person, the tests will take place online using the Teams and Elly platforms.